Seed Germination of Orchid | Research

Seed Germination of Orchid | Research There are a lot of orchid species being listed as endangered species due to over-collection and lack of conservation. The survival rate of orchid in the nature is relatively low. This research is aimed to investigate the effect of organic additives on in vitro seed germination of Vanda hastfera, an endemic species of orchid to Borneo. By the end of this investigation, we expect to determine the medium composition and germinating conditions that are favourable for in vitro seed germination of Vanda hastifera. Various organic additives such as potato extract, peptone, coconut water, tomato juice and banana pulp will be added into the medium to examine their effects on the seed germination. The physical conditions such as light intensity, temperature, pH and relative humidity will be adjusted to the most suitable condition in compliance with the percentage of seed germination. The culture room will be maintained at a 16 light/8 dark photoperiod with environmental temperature of 25 ±2à ‚ °C. Also, the protocol will be designed by taking into account of the cost-effectiveness of the project. Keywords: Vanda hastifera, in vitro seed germination, protocorm,  ½ MS medium 1.0 Introduction The family Orchidaceae is considered as one of the largest flowering plant families which comprised of over 880 genera and approximately 25,000 to 30,000 species worldwide (Paek, Hahn Park, 2011; Bektas, Cà ¼ce Sà ¶kmen, 2013). Paek et al. (2011) indicated that the high degree of compatibility among genera and species allowed the registration of bi- and plurigeneric hybrids to reach over the number of 100,000. Lamb (as cited in Chan, Lamb, Shim Wood, 1994, p. 5) claimed that approximately 10% of orchids in the world were found in Borneo, which is around 2500-3000 species. Among these about 30-40% are said to be endemic species. Borneo is whereby denoted as â€Å"Orchid Island† (Chan et al., 1994). As discussed by Beaman, Wood, Beaman and Beaman (2001), Vanda is a monopodial genus of orchids that produce attractive flowers which are generally found at hill-forests or tropical lowlands. Vanda hastifera Rchb.f. (Orchidaceae) is endemic to Borneo in which this epiphytic species inhabits at hill forest, lowland or coasted area (Chan et al., 1994). Vanda hastifera can be found at Kinabatang district in Sabah, Pontianak area in Kalimantan Barat, and Kuching area in Sarawak. Vanda hastifera is scented flower that can blooms for around 10 days in which it produces reflexed petals and sepals (Chan et al., 1994). The sepals of Vanda hastifera are marked by a few reddish brown spots. The uniqueness of this species is that it is very hairy at the margin of its lip as well as the auricles (Beaman et al., 2001). Over-collection of orchids has endangered some of the species which have high commercial value in a variety of industries like the medical industry, horticultural, ornamental so on. The survival of orchid in wild habitat is very low which also press the need to perform in vitro germination of the seeds. Vanda is one of the commonly cultivated genera that have high commercial value (Uchida, 1994). Based on the study that is fulfilled by Beaman et al. (2001), poorly preserved herbarium collections and insufficient information about the flower species for genus Vanda have made it substantial to practise cultivation upon the species. The significance in performing in vitro seed germination of Vanda hastifera is to optimize the seed germination process under in vitro condition in which the conditions and requirements of seed germination can be well-controlled. This can prevent the waste of resources and conserve the process of seed germination in making sure of the efficacy of the process . Furthermore, the addition of organic additives in appropriate concentration under proper circumstances can also enhance the effectiveness of seed germination carried out in vitro with relatively low expenses. The objectives of the study are: To investigate the effect of organic additives on in vitro seed germination of Vanda hastifera. To optimize the in vitro seed germination process of Vanda hastifera. To develop an appropriate and cost-effective protocol for in vitro seed germination of Vanda hastifera. 2.0 Literature Review 2.1 Vanda hastifera (Orchidaceae) According to Metusala and O’Byrne (2012), there are around ten taxa comprised in the genus Vanda section Hastifera which are distributed in the area of Borneo, Philippines, Lesser Sunda Island, Maluku, Sulawesi and New Guinea. The typical characteristics of section Hastifera are like complex lip with its apex divided into two buttock-like lobules and two dagger-like lobules bulging sideways from the base of the thickened mid-lobe (Metusala O’Byrne, 2012). Among the species of orchids in genus Vanda, Vanda scandens and Vanda hastifera have exhibiting all of the characteristics of section Hastifera. The name of Vanda hastifera is derived from Latin hastifer. Vanda hastifera is a fascinating species of orchid that has outstanding appearance which the fragrant flower for about 4.5 cm to 5 cm is fleshy and marked unevenly with brownish red blotches (Chan et al., 1994). Beautiful pale cream or pale yellow sepals and petals are reflexed. As revealed by Chan et al. (1994), the both the dorsal sepals and lateral sepals are spathulate with undulant margin. The white side lobes of the lip has shiny mid-lode at the top and side of the apex which joined to a short column-foot. The lower surface of the apex is purple to brownish purple with two reddish lines near the base. There is found to be a very hairy auricle at the base of the lip (Chan et al., 1994). Likewise, the edge of the mid-lobe is also hairy. The ligulate leaves of Vanda hastifera is about 15-20 Ãâ€" 2-2.5 cm which is dense and linked to a sheathing base. The embryo of Vanda differentiates into three sections in its early development, which are parenchymatous, meristematic and suspensor (Arditti, 1967). Based on the research done by Alvarez (as cited in Arditti, 1967, p. 4), the parenchymatous tissue is acting on supplying nutrition to meristem after the early degeneration of suspensor. 2.2 Orchid seed germination The orchid seed is very fine and delicate. The weight of the seed is varied from 0.3  µg to 14  µg; the length is to be at a range of 0.25 mm to 1.2 mm; and the width is around that of 0.09 mm to 0.27 mm (Arditti, 1967). The seed of orchid is normally produced in a large quantity in which Arditti (1967) indicated that a capsule may contains about 1,300 to 4,000,000 seeds. The orchid embryo usually maintains its globular or spherical shape in contrast to the great variety of the shape of the seed coat which may be in globular, elliptical, rounded, butterfly shaped or fusiform (Arditti, 1967). The swelling of embryo during germination will rupture the seed coat. This will lead to the formation of spherical or cone-shaped seedling which is the protocorm stage for orchid seed germination (Arditti, 1967). The protocorm is an undifferentiated mass of cells (McKendrick, 2000). Subsequently the first leaf primordium will project out of the upper flat surface. The protocorm then starts growing and the absorbing hairs starts to emerge at the periphery of the lower surface. Next, the first minute leaf is produced (Arditti, 1967). Soon after this, the first root will be formed. The development continues until a small plant takes it shape. In relation to orchid seed germination and development in nature, fungus infection is seemed to be a substantial factor for certain tropical epiphytic orchids (Arditti, 1967). This is because as the seeds are insufficient with the carbohydrate reserves, the young plant requires the supply of nutrients, sugar and organic material from the mycorrhizal fungus until the plant is capable of producing its own food (McKendrick, 2000). Once the fungus is penetrated into the seed, it is to be as an exogenous carbohydrate for the growing embryo upon the digestion of the fungal hyphae (Kauth, 2005). Moreover, fungi may be treated as a water supply as germination is started by imbibition (Yoder et al. as cited in Kauth, 2005, p. 2). 2.3 Research history of In vitro seed germination of orchid In vitro methods are used to improve and assist the development of plants that are vulnerable to grow in the nature (Fay, 1992). The use of symbiotic and asymbiotic in vitro germination techniques have been used for the seed germination of some orchid species (Fay, 1992). For the seeds that are to be germinated symbiotically, sowing is performed with a piece of mycorrhizal fungus. Symbiotic relationship is established when the fungus propagates and colonized the seed germination media (Mckendrick, 2000). Before the plant capable of making its own food, the fungus is thought to be sustaining the protocorm. Nonetheless, the proper strain of mycorrhizal fungus is required or else it might lead to seedling death as the fungus strain becomes parasitic. Proliferation of temperate terrestrial orchids is suitable to apply with this technique. On the other hand, tropical orchids are easier to grow as compared to temperate terrestrial orchids. Thus, asymbiotic germination method is normally us ed for the in vitro germination of tropical orchids. The media used appeared to be more intricate than that used in symbiotic germination (McKendrick, 2000). Without the mycorrhizal fungus, the nutrients required for proper germination have to be supplied fully. There are examples of simple media which are used for the seed germination of orchid which include Vacin and Went (VW), Hyponex and Knudson C medium (Paek et al., 2011). Without using the mycorrhizal fungus as a symbiotic element, Knudson (as cited in Kauth, 200, p. 3) has achieved successful seed germination for several epiphytic orchid genera which lead to the development of Knudson Solution B. After that, Knudson substituted ferric phosphate with ferrous sulphate and supplemented manganese into the medium in order to develop a more complex Knudson C medium that enable the in vitro seed germination and plant tissue culture suitable for more species (Kauth, 2005). Table 1: Media composition of  ½ MS, VW and Knudson media (Paek et al., 2011). Component  ½ MS (mg/L) VW (mg/L) Knudson (mg/L) Macronutrients NH4NO3 825 (NH4)2SO4 500 500 Ca3(PO4)2 200 Ca(NO3)2. 4H2O 1000 CaCl2.H2O 220 MgSO47H2O 185 250 250 KNO3 950 525 KH2PO4 85 250 250 Micronutrients Na2EDTA 18.65 FeSO4.7H2O 13.9 25 Fe2(C4H4O4).2H2O 28 H3BO3 3.1 CoCl2.6H2O 0.0125 CuSO4.5H2O 0.0125 MnSO4.4H2O 11.15 7.5 KI 0.415 Na2MoO4.2H2O 0.125 ZnSO4.4H2O 4.3 Organics Glycine 2 Myo-inositol 100 100 Nicotinic acid 0.5 1 Pyridoxine 0.5 Thiamine HCl 0.1 1 Adenine sulphate 10 The media composition is an important factor that will affect the efficiency of seed germination in vitro. MS medium added with 2,4-Dichlorophenoxyacetic acid (2,4-D) was reported to be favourable for the formation of protocorm-like-bodies (PLBs) and regeneration of plantlet for Dendrobium orchid (Nasiruddin et al. as cited in Aktar, Nasiruddin Hossain, 2008, p. 69). According to Mishra, Rawat, Nema and Shirin (2013), who have done an investigation on the effect of medium strength on in vitro germination of Pterocarpus marsupium Roxb. stated that by using different strength of MS basal medium, there is no significant difference on the rate of germination. Therefore, the concentration of MS medium used can be diluted to half so as to reduce the cost without affecting the efficacy of seed germination. Table 2: Modified Hyponex media composition (Paek et al., 2011). Component Seed germination Protocorm multiplication First transplanting Second transplanting Hyponex (g/L) N:P:K= 6.5:6:19 3.0 1.0 1.0 1.0 N:P:K= 20:20:20 1.0 1.0 1.0 Adenine Sulfate (g/L) 5.0 Peptone (g/L) 2.0 2.0 3.0 Coconut water (%) 20 10 10 Potato or banana homogenate (g/L) 30-100 30-100 Activated charcoal (%) 0.05 0.05 0.05 0.05 Germination of seed can be affected by seed maturity. By using asymbiotic in vitro seed germination technique, immature seed has found to be more effective than mature seed to germinate. Claiming that the embryos have developed completely but not yet dedicated to enter the dormant stage, Light and MacConaill (as cited in Fay, 1992, p. 2) suggested that seeds harvested at 43 to 58 days after pollination has found to be ideal for in vitro seed germination of orchid. 2.4 Effects of organic additives on in vitro seed germination Natural complex additives are added into the simple media lack amino acids or vitamins. Examples of organic additives are like potato extracts, coconut water, banana pulp, peptone, and tomato juice. Activated charcoal is believed to have ameliorated the aeration as well as absorbs ethylene and phenolic inhibitors which are the growth inhibitors. Nevertheless, Paek et al. (2011) suggested that the use of charcoal has to be careful as it also absorbs vitamins and plant growth regulators in the culture medium. Occasionally, low concentration of auxin and cytokinin are needed and supplemented into the media in the early stage of protocorm proliferation for certain species of orchids (Paek et al., 2011). Besides, sucrose is also being added into the media in the early stage of tissue culture. However, Paek et al. (2011) claimed that the plantlet differentiation of some genera of orchids may be improved in a medium which has low concentration of sugar. A research done by Islam, Akter and Prodhan (2011) which used Vanda roxburgii orchid as model has proven that by adding potato extract into the medium of seed germination in vitro, the percentage of seed germination and seedling growth can be increased substantially. From their study, they have found that by supplementing potato extract at the concentration of 200 ml/L showed the best percentage of seed germination for Vanda roxburgii which is 78.24% as compared to the 17.2% of control (Islam et al., 2011). Therefore, it is proposed by Islam et al. (2011) that seed germination of Vanda orchids can be boosted by adding appropriate concentration of potato extract into the medium. It is testified that in 100 g of raw materials of potato extract, there is 1.0 mg of niacin which is believed to be accountable for the orchids’ development. Recent study accomplished by Tharapan, Thepsithar and Obsuwan (2014) has observed the effect of potato extract (PE), soy milk, cow milk and peptone on the development of Dendrobium discolor’s protocorms and seedlings growth of Dendrobium Judy Rutz by using Hyponex medium. After two months, protocorms were found developed in all culture vessels with different combinations of organic supplements in Hyponex medium. For the seedling growth of Dendrobium Judy Rutz, Hyponex medium with supplementation of 100 mL/L of potato extract and 2.0 g/L of peptone has achieved with the maximum fresh and dry weight (Tharapan et al., 2014). Conversely, the dry weights obtained from Hyponex medium supplemented with peptone, organic soy powder and milk powder for Dendrobium Judy Rutz’ seedling growth have no significant difference in comparison to the control. On the other hand, the maximum fresh and dried weight as well as shoot height of Dendrobium discolor’s protocorms were obtain ed in Hyponex medium containing potato extract with 2.0 g/L peptone (Tharapan et al., 2014). 3.0 Materials and Methods 3.1 List of Materials Seeds of Vanda hastifera, 0.2% (w/v) HgCl2, 70% ethanol, 1% 2,3,5-tripheny tetrazolium chloride (TTC), MS medium, KC medium, Hyponex medium, coconut water, potato extract, banana homogenate, peptone, yeast extract, tomato juice, 0.9% (w/v) agar, sucrose, activated charcoal, NaOH and HCl. 3.2 Sterilization of the seeds Immature seeds of Vanda hastifera is surface sterilize by submerging in 0.2% (w/v) HgCl2 for 10 minutes. After this, the seeds will be dipping in 70% ethanol for 15 seconds for further sterilization. Sterilized seeds are then washed with sterile distilled water for 5 to 6 times. These procedures are operated in a laminar flow hood. The viability of the seeds is examined by staining the seed with 1% 2,3,5-triphenyl tetrazolium chloride (TTC) solution (pH 7). The staining is carried out in darkness. Observation is performed by using stereoscopic microscope (Yamazaki Miyoshi, 2006). Viable embryos are those shows orange to red colour under the stereoscopic microscope observation (Lauzer, Renaut, St-Arnaud Barabà ©, 2007). This procedure has proven effective for testing the viability of epiphytic tropical orchid’s seeds (Singh as cited in Vujanovic, St-Arnaud, Barabà © Thibeault, 2000, p.79). 3.3 Media preparation Half and full strength Murashige and Skoog (MS) medium (Murashige and Skoog, 1967), KC medium (Knudson, 1946) and Hyponex medium (Kano, 1965) are supplemented with various organic additives in different concentration to examine their effects on in vitro seed germination of Vanda hastifera. Coconut water (5, 10, 15, 20% v/v), peptone (0.2% w/v), yeast extract (0.2% w/v), potato extract (1, 2, 4, 6 g/L) (w/v), banana homogenate (2.5-12.5% w/v), tomato juice (10-20% v/v) are added into the medium. 30 g/L of sucrose and 2 g/L or activated charcoal are also supplemented into the medium. The medium is solidified with 0.9% (w/v) agar. The pH of the medium is adjusted by using NaOH or HCl to between 5.4 to 5.8 prior to autoclave at 121 under 15 p.s.i. for 20 minutes. (Jawan, Gansau Abdullah, 2010; Ali, Murdad Latip, 2011) 3.4 Inoculation The sterilized seeds are then inoculated on to the medium prepared. The spreading of the seeds is to be even over the entire surface of the medium. The Petri dishes with media inoculated with seeds are kept in the culture room provided with 16 light /8 dark hours of photoperiod at 25 ±2. The light intensity is to be at 20-50  µmolm-2s-1 provided by cool white fluorescent tubes (Jawan et al., 2010; Ali et al., 2011). The relative humidity is adjusted at 70-80%. 3.5 Observation and analytical technique Seed germination is indicated by the development of protocorms. According to Ali et al. (2011), it can be seen by the appearance of protocorm from the testa. Observation is carried out at a 20 days interval for up to 60-70 days by using a dissecting light microscope. The number of germinating seed is recorded and tabulated in a table as the percentage of the total number of seeds inoculated (Ali et al., 2011). Analysis of variance (ANOVA) is used to analyse the data. The significant difference or treatment means is subjected to Duncan’s Multiple Range Test (DMRT) at 5% level of probability (Aktar et al., 2008; Ali et al., 2011). 4.0 Expected outcome The seed germination of Vanda hastifera that is conducted in vitro will be very effective. It is greatly affected by the medium composition, concentration of organic additives supplemented and the light intensity. It is expected that the  ½ MS will be the best medium for in vitro germination of Vanda hastifera. Organic additives like potato extract, peptone and coconut water are expected to give better results in the experiment as compare to other organic additives.

Analysis Of Sonnets 64 And 73 Essay

William Shakespeare is one of the greatest playwrights of all time. It is also important, however, to remember and to study his sonnets. The sonnets are separated into two groups, 1-126 and 127-54. All of them are love poems of some sort, whether addressed to a young man or the infamous â€Å"Dark Lady.† It is important to compare and analyze the sonnets, and to see the similarities between them. The purpose of this essay is to compare sonnets 64 and 73, and show that although it is easy to come to the conclusion that they are sorrowful in tone and negative in orientation, they are truly positive and life affirming. These two have been chosen because they are similar in this and other respects. Before discussing the similarities, however, it is necessary to briefly describe what each sonnet is about. Sonnet 64 is a cry against the inevitable arrival of all that wears down even the most firm powers that exist in the world. The speaker stresses that even the most sturdy monuments are bound to the ravages of time: â€Å"When I have seen by Time’s fell hand defaced/ The rich, proud cost of outworn buried age,/ When sometime lofty towers I see down-raz’d/ and brass eternal slave to mortal rage;† and so on. It is clear that the speaker finds time an enemy, capable of eroding any efforts to persevere. Time is also the enemy to the desire to be with a loved one forever. In this sonnet, the speaker finds himself at the mercy of his opponent, without any means of facing Time with any success. He almost abandons the love that he feels because he knows that it will eventually fall victim to time. There is no difference between the love that is felt by the speaker and the other durable things in the world, such as the â€Å"kingdom of the shore†, and the â€Å"firm soil.† But even these things will erode over time. The only option the speaker has is to mourn what he will one day lose. The seventy-third sonnet is also about the response of the speaker to the fact that Time detracts from the endurance of man and his response to the things that make him feel loved. Shakespeare starts with a discussion of the process by which the things that surround man first start to erode and fall as a result of the passing of time. The speaker is equating himself to  autumn and the twilight of day. He finds himself lying on the ashes of his youth, and a victim to the passage of time. He cannot sustain the love that he feels, and is consumed by both time and love, as they once sustained him. The speaker is arguing that the fate of man is to be consumed by the very things that are his life-blood: love and time. â€Å"In me thou seest the glowing of such fire/ That on the ashes of his youth doth lie,/ As the deathbed whereupon it must expire/ Consumed with that which it was nourished by. The similarities between these two poems are evident. Basically, they are both about the speaker’s sense of helplessness and loss in the face of the passage of time. The theme of loss, and the recurrent theme of impotence when faced with passing time and its effects, is evident in both poems. However, these are not necessarily sad or defeatist poems. The speaker does not submit to the passage of time by saying that he will not be able to feel or love or even live anymore. He is not depressed to the point of being unable to do anything. Rather, the speaker feels that man must continue to love, and to live, despite the fact that life will end, and love will eventually subside as time takes over the human spirit. Although â€Å"Time will come and take my love away,† the speaker is not saying that man must simply not love at all. He is saying that man must eventually give in to the effects of time, but that in the time that does exist for man, it is possible to love, and to sustain oneself with that love. These poems, which sound sad or even lacking in spirit, are actually affirmative of the desire toward love and life: â€Å"This thou perceivest, which makes my love more strong,/ to love that well, which thou must leave ere long.† Both of these sonnets can be interpreted as encouraging the reader to grasp the fact that love can be sweeter and more enduring if the individual realizes that time will eventually take that love away. It is even possible to claim that, because all love will end, man should state his love early, and live that love to the fullest extent possible. In this sense, each of these poems can be understood to be positive, and life affirming. At first reading, it is easy to come to the conclusion that the poems are  sorrowful in tone and negative. However, after closer analysis, it is obvious that the speaker is ultimately celebrating life, and urging the embrace of all aspects of it, whether they result in suffering or pleasure. The tone is sorrowful when the speaker comes face to face with the inevitable, but the fact remains that the inevitable outcome, which is loss, and the passage of time, is part of what makes the intensity of love, and the quality of life, so memorable and so pleasurable.

Essay on Motivation and Religion - 1064 Words

Motivation and Religion According to Dr. David Elkins, Contemplation, meditation, prayer, rituals and other spiritual practices have the power to release the life force in the deepest levels of the human psyche, levels that secular interventions cannot reach (Elkins 1999). Spirituality may not just about one person healing. It may be about the look of wonder on a childs face or the love we feel for a family member. It could also be about finding the place that attaches one person to another. Its about seeing the sacred in our lives and opening the door to a life of passion and depth or even strength and power. There is no right or wrong answer. To each their own, but there must be something that motivates each and†¦show more content†¦That could definitely make one embrace another religion. A major role in embracing a religion or spirituality has to do with internal motivation. Anger, fear, and/or hurt could motivate someone to seek out those unlike and uncomfortable feelings and find happi ness, love, and security in religion. Someone could easily find a belief that gives him/her hope. People in general define their god, not as a person, but as a force, a principle, . . .an integrating power that expresses the unity and the meaningfulness of a higher-being (Maslow 1976). Some recognition needs to be given to factors which are basic to an understanding of any religious experience. [Religious] attitudes in general are born of conditioning circumstances which have been more or less persistently present in the formation of the psycho-physical personality. Man gets his attitudes from longstanding associations with parents, social groups, intellectual constants; in short, with those environmental influences of longstanding (Olt 1956). A second factor in religious experience is the acquired sentiments about religious things. 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Chiles Culture: In the textbook , â€Å"An Introduction to Intercultural Communication: Identities in Global Community,† by Fred E. Jandt, the author defines culture as the, â€Å"sum total of ways of living, including behavioral norms, linguistic expression, styles of communication, patterns of thinking, and beliefs and values of a group large enough to be self-sustaining and transmitted over the course of generations.† In a cultureShow MoreRelatedA Educational Excellence And Standards For More Than 30 Years Essay1545 Words   |  7 Pagesmany students from all over the world, creating an environment full of cultural variety. RIT has helped to the economy from those countries and other such as the Dominican Republic, preparing professional to make a successful career in a global society. RIT has been focused on expanding its campuses in countries where they know could have a great impact in today’s global economy development. 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He first stayed only in Central and South America, but soon decided that he wanted to go further (Matta:Read MoreMarket Trends And Developments Of The Salmon Export And Export Business Essay1187 Words   |  5 PagesBSBMKG507 Interpret market trends and developments Assessment Task 2: Project – Market Analysis Salmon Import and Export Business Market development The history of salmon farming can be traced back to fertilization testing in Europe in the second half of the eighteenth century. Hatcheries were set up one century afterward in both Europe and North America. Hatchery-based enhancement programs were introduced at a large scale only after the 1950s in Japan, Russia, USA and Canada. The modern systemsRead MoreObesity : Obesity And Obesity989 Words   |  4 Pagesto recent analysis, statistics show that more than one third of the world’s population is targeted by obesity. Research shows that overweight and obesity kills more people than underweight. Obesity occurs greatly amongst adults who are 18 years or older. An estimated 600 million adults were obese out of the 1.9 billion that were overweight, around the world in 2014. One in three adults in Mexico, New Zealand and United States are obese, and one in four adults in Australia, Canada, Chile and HungaryRead MoreA Free-Trade Agreement Between the US and Europe1517 Words   |   7 Pageschallenges in doing so. b) Chile and Argentina Argentina is one of the top vegetable oil producers in the world-due to low domestic consumption and high productivity- with Chile as a main buyer. After a tariff reclassification in 1999, the Chilean price band system (PBS) resulted in higher customs duties of up to 64.41%, thus violating the limit set at 31.5%. As a result, this issue was submitted to the WTO . c) U.S and Europe A free-trade agreement between the United States and Europe has been elusiveRead More Women in the Developing World Essay1049 Words   |  5 Pagesplay an important role in the modernization process. Third world women, just as woman in industrialized nations, are largely represented in particular occupations. The majority work in agricultural employment or jobs that are unregulated by the state, such as street vendors and small businesses. Similarly, as in industrialized nations, Third World professional women are over-represented in such professions as nursing and teaching. Divisions between women’s and men’s work have obvious economic andRead MoreSouth Korea An Attractive Market For Australian Beef1344 Words   |  6 Pagesbeef market, and whether it is an attractive market for South Korea to import from. An analysis of the current Australian and South Korean beef industries; the conditions required to produce beef most efficiently, following the Heckscher-Ohlin Model; tariffs that are involved with these trades, and the impact of the Korea-Australia Free Trade Agreement; environmental implications to Australia; as well as the cultural environment and geographic factor, will be discussed to determine whether South KoreaRead MoreEducation is key to knowledge that strengthens the perspectives, skills, and abilities of learners1500 Words   |  6 Pagestransportation opportunities, poor technology, crammed schools, little individual attention and support by teachers and or parents (Frankema 2008). Health problems also affect children’s abilities to take opportunity of primary education (Frankema 2008). Cultural and economic expectations also negatively affect many kids, as girls may be expected to do domestic work and boys to do outside labor; child labor take educational opportunities away (Torche 2010; Frankema 2008). Also, many governments in Latin AmericaRead MoreDelta Airlines Contact Center : Analyisis of the Country Options3065 Words   |  13 PagesTHE DISCUSSION 6 THREATS 6 SOLUTION 9 CONCLUSIONS 10 RECOMMENDATIONS 11 BIBLIOGRAPHY 12 Introduction Delta Air Lines, Inc. (Delta) is an air carrier that provides scheduled air transportation for passengers and cargo throughout the United States and around the world. The Company offered customers service to destinations with Delta and Delta Connection carrier service to 321 destinations in 58 countries in January 2008. To Latin America and the Caribbean, Delta offered more than 500 weeklyRead MoreAn Analysis of Pablo Neruda’s The United Fruit Co. Essay1415 Words   |  6 PagesNeruda is from Chile and gives a voice to Latin America in his poetry (Bleiker 1129). â€Å"The United Fruit Co.,† the poem by Pablo Neruda that will be analyzed in this essay, is enriched with symbolism, metaphors, and allusions. These allusions have great emphasis to the Christian religion, but some allusions are used to evoke negative emotions towards the United States (Fernandez 1; Hawkins 42). Personification and imagery along with onomatopoeia and metonymy are also found in â€Å"The Unit ed Fruit Co.† Neruda’s

Memories And The Formation Of Reality - 1666 Words

In cinema, flashbacks are interruptions that take the narrative back in time from the current point in the story. They are often used to provide background and context to recount current events of a narrative filling in crucial backstories. In its basic form, the flashback is introduced when a presented image dissolves to another image of the past, which can be either as â€Å"a story-being-told or a subjective memory.† (Turim) For example, dream sequences and memories are methods used to present flashbacks. They become a visual representation to the audience as an act of remembering past experiences of reality. Imagination also plays an important role in shaping the mind and the story, similar to memories and the formation of reality. Imagination is usually integrated into animation as an artistic creation to express the artists’ ideas or memory. But where does the use of imagination and memories stop? By examining memory represented in animation and film, through Bart lett’s theory of schema, we can see how the film develops an active organized method to distinguish reality from memory. This paper examines five films based on memory, thus arguing that imagination does not distort memory in animation, but create a role as a form of embellishment for the film to help memory. Fredrick Bartlett is a British psychologist from Cambridge University, based on his book, Remembering (1932) that focused on how people comprehend what was being remembered. The concept of schema orShow MoreRelatedMemories And The Formation Of Reality1688 Words   |  7 Pagesstory-being-told or a subjective memory.† (Turim, pg. 1) For example, dream sequences and memories are methods used to present flashbacks. They become visual representation to the audience as an act of remembering past experiences of reality. Imagination also plays an important role in shaping the mind and the story similar to memories and the formation of reality. Imagination is usually int egrated into animation as an artistic creation to express the artists’ ideas or memory. But where does the use ofRead MoreMemory Of Memory And Memory966 Words   |  4 PagesOne of the common issues today is if people can rely on their memories. It is not mutual knowledge for people to know how memories are formed or where they are stored. A prodigious example of how memories are constructed is in the article â€Å"Distinguishing the Neutral Correlates of Episodic Memory Encoding and Semantic Memory Retrieval† Steven Prince and the other authors report, â€Å"The way a memory is formed is by neurons in one’s brain retaining information from a certain event and delivering it toRead MoreThe Secret Window And Dissociative Identity Disorder1096 Words   |  5 Pagesof Dissociative Identity Disorder, however, is the presence of two or more distinct identities that alternately take control of the individual and this symptom is the main basis and plot of The Secret Window. In addition, the person is plagued by memory losses that cannot be explained by ordinary forgetfulness and may even find items that he or she doesn’t remember buying. (â€Å"Dissociative Identity Disorder (Multiple Personality Disorder)†, 2014). Also, the individual may experience unexplained changesRead MoreThe Effects Of Social Priming On Memory Formation1627 Words   |  7 Pages The Effect of Social Priming on Memory Formation Isabel Rauchle Psy 110 - McCoy Nov. 30, 2016 In today’s society, and many generations before us, stereotypes have overrun how we think as a collective group. From the way we act around others to the way we perceive the actions and conversations around us, they are all influenced by stereotypes. Whether these stereotypes are taught to us from our parents, our peers, or through media influencesRead MoreDefense Mechanisms Are The Way Someone Distances Themselves From A Full Awareness Of Unpleasant Thoughts, Feeling, And1213 Words   |  5 Pagesregression, acting out, dissociation, compartmentalization, projection, reaction formation, repression, displacement, intellectualization, rationalization, undoing, sublimation, compensation, and assertiveness. The last few are known as mature defense mechanisms. They are the most constructive and helpful for most adults, however they require practice and effort to use in daily use. Denial is the refusal to accept a fact or reality. It is the most common of defense mechanisms because it’s a characteristicRead MoreThe Ego as a Defense Mechanism Essay730 Words   |  3 Pagesrealistic dangers. Anna Freud lists nine defence : REGRESSION, repression, REACTION FORMATION, ISOLATION, UNDOING, PROJECTION, INTROJECTION, TURNING AGAINST THE SELF, and REVERSAL - plus tenth SUBLIMATION. SPLITTING and DENIAL are also usually listed as defence. It is usually assumed that defence belong to specific stages of development, e.g. INTROJECTION , projection, denial , splitting to the ORAL phase; reaction-formation , isolation and undoing to the ANAL phase. Defence MechanismsRead MoreGlobalization : Identity Formation And Globalization906 Words   |  4 PagesAaron Altier Dr. Eleanor Latham Writing 121 2 March 2016 Globalization: Identity Formation Globalization can be defined as the â€Å"process by which different parts of the world become interconnected by economic, social, cultural, and political means† (Globalization 2015). It affects every aspect of our lives, and we experience globalization daily when we go to the grocery store and view products and produce from other countries, when in coffee shops we are surrounded by faces and languages from otherRead MoreDefense Mechanisms, Freud1600 Words   |  7 Pagesmechanisms include repression and denial, which serve to prevent unacceptable ideas or impulses from entering the conscience. Secondary defense mechanisms-generally appearing as an outgrowth of the primary defense mechanisms-include projection, reaction formation, displacement, sublimation, and isolation(AllPsych). Freud introduced the idea that the mind is divided into multiple parts, including the irrational and impulsive Id (a representation of primal animal desires), the judgmental super-ego (a representationRead MoreThe Nothingness Of Personality By Jorge Luis Borges1309 Words   |  6 Pagesas the private possession of some depository of memories is mistaken† (1). However, I would argue that memories do in fact play a substantial role in developing human personalities, and that without memories, our self and personality is incomplete. While Borges is correct in stating that our memories are fleeting and that one never completely remembers an event in its entirety, the feelings and emotions accompanying such event impact the formation of our identities. While life events certainly areRead MoreThe Life And Works Of Sigmund Freud Essay1679 Words   |  7 Pageswas born in Moravia hamlet of Freiberg, Germany on May 06, 1856 and died in London, England on September23, 1939. He was bor n in the era of â€Å"logic and reason† (Love and the formations of family) when sciences was all that mattered and rational analysis was perceived as being the answer to all â€Å"mysteries† (Love and the formation of family). At the age of three his family relocated to Austria where he lived until the last year of his life (World of health, 2007). He was admitted into the medical school

Not My Best Side Free Essays

How does the lanuage used create effects? The following paragraphs explore how language is used to create effects in the poem Not my Best Side by U. A. Fanthorpe, an ekphrastic poem that uses the free verse form arranged in three stanzas of nineteen lines each. We will write a custom essay sample on Not My Best Side or any similar topic only for you Order Now It’s purpose is to entertain, which it does through the use of humour, using Standard English in an informal, conversational manner. Dramatic monologues from the characters re-tell from their own perspective, the historical myth of a brave knight that slays a fearsome dragon and rescues a damsel in distress. There are some differences however, and playing with the readers expectations and using modern stereotypes in place of the historically accepted character roles is where much of the humour is derived. the dragon, unlike it’s mythological counterpart has quite a sophisticated vocabulary, use of adjectives like ‘ostentatiously beardless’ and the idiom ‘old chap’ suggests an intelligent, well spoken, stereotypical British, Upper Class gentleman, the antithesis of a ferocious monster. The line ‘Not my best side, I’m afraid. , reveals the dragon’s self conciousness and obsession with appearance, a reocurring theme. The nouns ‘artist’ and ‘pose’ indicate the poem is about a character in a painting and the dragon is highly critical of both the painter, ‘Poor chap, he had this obsession with Triangles, so he left off two of my Feet. ‘ and the other characters, ‘Why should my victim be so Unattrac tive as to be inedible,’. It is the comical and conversational tone that makes the dragon so likeable and lots of rhetorical questions draw the reader in, particularly ‘(What, after all, are two feet To a monster? ‘ which, enclosed in brackets gives the impression the dragon is addressing the reader directly. Continuing the pattern of reversing the stereotypes, the girl is presented as a slightly feminist, with a mind of her own, ‘It’s hard for a girl to be sure if She wants to be rescued. ‘, not the typical damsel in distress and likewise, not the naive maiden expected in this kind of myth, euphemisms such as, ‘He made me feel he was all ready to Eat me. And any girl enjoys that. ‘ and ‘the dragon– Well, you could see all his equipment At a glance. are quite crude sexual innuendo of a sexually aware individual. She is a modern, young woman indicated by the nouns she uses, ‘hardware, acne, blackheads’, and i s just as obsessed by appearance as the dragon, ‘He was So nicely physical, with his claws And lovely green skin, and that sexy tail,’ it is interesting that her opinion of the dragon is much more flattering than his opinion of himself. It is also ironic in view of the dragons opinion of her, she thinks the dragon fancies her ‘And the way he looked at me,’. Perhaps she is still a little naive, and the final lines of the second stanza, ‘Still, what could I do? The dragon got himself beaten by the boy, And a girl’s got to think of her future. ‘ suggests a certain helplessness, as though there really is no escape from the role assigned to her by society, despite her own desires. The final stanza opens with a very funny line, ‘I have diplomas in Dragon Management and Virgin Reclamation. ‘, especially if one takes a moment to consider the training involved in ‘virgin reclamation’. It suggests the knight has recently completed his training and is quite young, perhaps his first day on the job. Despite the humour, he is nothing like the chivalrous knights of legend. He is arrogant, ‘You can’t Do better than me at the moment. ‘ Clearly a ‘product’ of the modern age, and obsessed with gadgets, ‘My horse is the latest model ‘, Much of his language is from a modern lexicon, ‘management, reclamation, prototype’ etc and from the semantic field of modernity, ‘contemporary, latest, and at the moment’ all of which place him in the present rather than the era in which this myth has it’s roots. He seems a little confused when confronted by the girl’s modern, feminist attitude, ‘Don’t You want to carry out the roles That sociology and myth have designed for you? ‘. He repeats the words ‘Don’t you†¦? ‘ three times, deciding chauvinistically, what the girl wants is irrelevant, ‘What, in any case, does it matter what You want? You’re in my way. ‘. How to cite Not My Best Side, Papers

How was Tension and Drama created in the Heck

How was Tension and Drama created in the Heck-Tate scene of To Kill A Mockingbird? Essay Tension and Drama is created in this extract using various methods, form body language to the entire situation. To begin, the average sentence length of this has great effect on the tension and drama created in this passage. For example Mr. Tate uncrossed his legs and leaned forward. This sentence is long and descriptive, this gives a relaxed and controlled image of the situation, whereas if the sentence had been short and sharp the pace of the extract would be much quicker, and after a few of this type of sentence the reader would begin to feel that the first person was experiencing a loss of control. Another aspect, which contributes to the tension and drama in the passage, is the situation. The reader knows this because Scout says Atticus could blandly introduce me to Boo Radley at a time like this, the situation being that Boo Radley has appeared for the first time in x-many years, after much fantasy on behalf of Scout and Dill. This is tense because it makes the reader has heard so much about this figure and now they are seeing him, not just seeing him in fact; but the reader are also under the impression that it was he who killed Bob Ewell. Another great participant to the tension in this passage is the pace of the text, when Heck-Tate tries to explain to Atticus that Jem didnt kill Bob the pace increases significantly. A good example of this is the short phrase I wont have it, this increases the pace because it is short. This shows that the pace of the text also shows how tension and drama is created. Body language also plays a significant role in creating the tension and drama in this passage. For example, Mr. Tate spat into the shrubbery, then thrust his hands into his hip pockets, is a good example of how body language effects the tension and drama. After this quote Atticus also mirrors this, the end effect begin not just that Mr. Tate is frustrated, but also that Atticus is trying to say the same, in effect creating a stand-off between the two. Thus, creating the drama through the body language. An additional method the author uses to create tension and drama is the weather, this is dramatic because it is night-time. The reader can tell this because Scout narrates the light from the living room windows was strong on them, the lights in the living room would not be on if it was day time. This is used in such a way that it sets the scene for slow the pace, but more importantly it makes the occasion that Boo Radley does appear seem more usual, as he has only been ever known to surface in the night. Therefore, creating tension. Most probably the best example of methods for creating tension and drama expressed in this passage is the extreme emotion. For example God damn it, Im not  thinking of Jem! This is extreme emotion because Heck-Tate is shouting at Jem, the only time somebody shouts in the book, because Atticus thinks that Jem killed Bob Ewell, when it is obvious to the reader that this is complete fallacy. It is tense because of the way that Heck-Tate is desperately trying to explain to Atticus that Jem is innocent and that Boo killed Bob, even though he cant say this. My conclusion to this is that it is a combination of these factors, which create tension and drama in this passage.